Step 1 manual
Step 2 PCR
- 96 well template plate with DNA pipette pcr master mix into all wells w/ Mantis 19uL stamp template plate into 96 sample well plate 1uL final vol 20uL then put into thermocycler for PCR then manual E-gel (QC)
Step 3 Kingfisher just run it
- NOTE: 5 plates for kingfisher use
- 1 sample plate (fresh) → output
- 1 kingfisher plate (magnets) → input
- 2 70% ethanol
- 1 elution
- trash plate
Step 4 CFPS Sample plate comes in (20uL) new cfps master mix has to be produced (manually) pipette cfps master mix into all wells w/ Mantis 14uL stamp template plate into 96 sample well plate 1uL final vol 15uL into BioRad for 30C for 6hours, then 4C overnight
Step 5 SPR some liquid handling, then put the plate in.
https://docs.google.com/document/d/12u4QJCgdLYibKAtfCeOSfJqOY-l4dZDX/edit https://app.notion.com/p/medra-ai/Chestnut-Assays-3b21b2bda0bb80deadaac9efa0c5e369?source=copy_link
to do tomorrow: assemble protocols